B-hBCMA/hGPRC5D MC38_Biocytogen Pharmaceuticals (Beijing) Co., Ltd._Biocytogen, drug development, antibody discovery

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B-hBCMA/hGPRC5D MC38

Common name	B-hBCMA/hGPRC5D MC38	Catalog number	322252
Aliases	BCM, BCMA, CD269, TNFRSF13A; /	Disease	Colon carcinoma
Organism	Mouse	Strain	C57BL/6
Tissue types	Colon	Tissue	Colon

Description

Origin: The MC38 cell line is derived from C57BL6 murine colon adenocarcinoma cells. The cell line is a commonly used murine model for colorectal carcinoma.
Background Information: In multiple myeloma (MM), BCMA and GPRC5D are significant immunotherapeutic targets. BCMA, a type III transmembrane domain protein of the tumor necrosis factor receptor superfamily, is encoded by the TNFRSF17 gene on chromosome 16p. On the other hand, GPRC5D is an orphan 7-pass transmembrane receptor protein encoded by the GPRC5D gene on chromosome 12p. Therapies involving anti-BCMA or anti-GPRC5D CAR T and TCE have shown promising results in treating relapsed and refractory MM.
Gene targeting strategy: The human BCMA coding sequence was inserted into the Rosa26 locus in MC38 cells. The mouse Gprc5d gene was replaced by human GPRC5D coding sequence in B-hBCMA/hGPRC5D MC38 cells.
Tumorigenicity: Confirmed in C57BL/6 mice.

Application: B-hBCMA/hGPRC5D MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies.

Notes:

Before implementing the project, it is recommended to perform tumor growth experiments. The recommended cell inoculation amount is between 5E5-5E6.

It is recommended to choose anti-human BCMA antibodies with good specificity for protein expression analysis, such as Belantamab analog.

Protein expression analysis

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BCMA and GPRC5D expression analysis in B-hBCMA/hGPRC5D MC38 cells by flow cytometry. Single cell suspensions from wild-type MC38 and B-hBCMA/hGPRC5D MC38 cultures were stained with species-specific anti-BCMA antibody (in house) and anti-GPRC5D antibody. Human BCMA and GPRC5D were detected on the surface of B-hBCMA/hGPRC5D MC38 cells. The 3-D03 clone of B-hBCMA/hGPRC5D MC38 cells was used for in vivo tumor growth assays.

Tumor growth curve & body weight changes

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Subcutaneous homograft tumor growth of B-hBCMA/hGPRC5D MC38 cells. B-hBCMA/hGPRC5D MC38 cells (5x10⁵; 1x10⁶; 5x10⁶) and wild-type MC38 cells (5x10⁵) were subcutaneously implanted into C57BL/6 mice (female, 8-10-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm³ using the formula: V=0.5 X long diameter X short diameter². As shown in panel A, B-hBCMA/hGPRC5D MC38 cells were able to form tumors in vivo and can be used for efficacy studies.

Culture method

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Note: If antibiotic maintenance pressure screening should be added, do so after stable cell growth.