| Strain Name |
|
Stock No. |
110124
|
| Common name |
B-Dmp1-iCre mice
|
Gene symbol and name |
Dmp1 dentin matrix protein 1
|
| Strain of origin |
C57BL/6 |
Chromosome | 5 |
| Coat color | Black | Dietary information |
Growth and reproduction diet for experimental mice |
Results of agarose gel electrophoresis
Gel image of PCR products. PCR products are separated by gel electrophoresis on a 2% agarose gel. PC: samples used for PCR were collected from heterozygous mice; WT: samples used for PCR were collected from wild type mice; H2O: blank control.
Phenotype Analysis
NPY deletion in osteocytes rather than in osteoblasts of Dmp1-iCre (Dmp1-iCre was purchased from Biocytogen Co. Ltd., Beijing, China); Npyfl/fl mice, was confirmed by western blotting for the protein lysates of the isolated osteocytes and osteoblasts (A).
NPY protein expression was much lower in the bone marrow-depleted femur homogenates, but not in the whole bone marrow cells, the whole brain lysate, and the NPY-abundant hypothalamus, of 4-month-old Dmp1-iCre; Npyfl/fl mice than that of Npyfl/fl littermates (C).
(source: Yan Zhang, et al., 2021)
