| Strain Name |
|
Stock No. |
110122
|
| Common name |
B-Col10a1-iCre mice
|
Gene symbol and name |
Col10a1 collagen, type X, alpha 1
|
| Strain of origin |
C57BL/6 |
Chromosome | 10 |
| Coat color | Black | Dietary information |
Growth and reproduction diet for experimental mice |
Results of agarose gel electrophoresis
Gel image of PCR products. PCR products are separated by gel electrophoresis on a 2% agarose gel. PC: samples used for PCR were collected from heterozygous mice; WT: samples used for PCR were collected from wild type mice; H2O: blank control.
Phenotype Analysis
Col10-Cre-marked cells, which excluded periosteal cells, gave rise to ~58%of all Lepr+ BMSCs.
(C) Confocal imaging of femur sections from Col10cre; R26tdTomato mice at P3 showed that Col10-Cre (Col10-Cre was purchased from Biocytogen Co. Ltd., Beijing, China) marked cells included chondrocytes, but not periosteal cells. Asterisks indicated the periosteum. (n=3 mice from 3 independent experiments)
(D and E) Confocal imaging (D) and flow cytometry (E) showed that Col10-Cre marked cells gave rise to most of the Lepr+ BMSCs at 6 months of age. (n = 3 mice from 3 independent experiments)
(source: Hui Sophie Shu, et al., 2021)
