| Strain Name |
|
Stock No. |
110137 |
| Common name |
B-Alb-iCreERT2 mice
|
Gene symbol and name |
Alb, albumin; Alb1; Alb-1; BCL002 |
| Strain of origin |
C57BL/6 |
Chromosome | 11 |
| Coat color | Black | Dietary information | Growth and reproduction diet for experimental mice |
|
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Phenotype Analysis
B-Alb-iCreERT2 mice were crossed with B-CAG-LSL-tdTomato mice. The experimental mice are injected with tamoxifen at an age of 4–6 weeks. Inject each mouse intraperitoneally (i.p.) with 80mg/kg of tamoxifen or with vehicle for five consecutive days. Mice were analyzed 1 week after the last injection.
Analysis of liver by immunofluorescence (IF).
Control: without tamoxifen treatment; HNF4α: a regulator of liver-specific gene expression. Immunofluorescence for HNF4α, tdTomato, and DAPI on liver sections shows Alb-iCreERT2(Mut/+), CAG-LSL-tdTomato(Mut/+) mice labeled almost all hepatocytes after tamoxifen treatment.
Analysis of kidney by immunofluorescence (IF).
Control: without tamoxifen treatment; HNF4α: a regulator of liver-specific gene expression. Immunofluorescence for HNF4α, tdTomato, and DAPI on kidney sections shows Alb-iCreERT2(Mut/+), CAG-LSL-tdTomato(Mut/+) mice were not labeled after tamoxifen treatment.
